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For example, homocysteine (Hcy), an amino acid involved in DNA methylation, facilitates GPX4 methylation that leads to upregulation of oxidative stress and ferroptosis in nucleus pulposus (Zhang et al., 2020c)

Concurrently, GSH reduces Fe 3 + to Fe 2 + , which activates S 2 O 8 2 to generate highly toxic sulfate (SO 4 ) and hydroxyl radicals, inducing coordinated ferroptosis and pyroptosis in lung cancer cells 219

Chromatographic Separation: A reverse-phase C18 column with a gradient elution profile was used to separate GSH and GSSG from other metabolites

doi: 10.1038/s41590-021-00996-0 98 WangYTianQHaoYYaoWLuJChenCet al