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An animal study in a reserpine-induced fibromyalgia rat model found that nano-pregabalin (N-PG) alleviated FM symptoms by enhancing CNS penetration, restoring neurotransmitter balance (Glut, NE, CGRP), increasing antioxidant enzyme activity (e.g., SOD), inhibiting pro-inflammatory transcription factors (e.g., NF-B), and reducing apoptosis by decreasing caspase-3 (Casp-3) activation (36)

These compounds are categorized as class 2 ferroptosis-inducing compounds (FINs)

Construct The human GIPR DNA (Genewiz) with one mutation (T345F) was cloned into the pFastBac vector (Invitrogen) with its native signal peptide replaced by the haemagglutinin (HA) signal peptide