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Nutrients 8(12):E798 Funding This study was supported by a grant of University of Perugia (Fondo di finanziamento della ricerca di base del Dipartimento di Scienze Chirurgiche e Biomediche, year 2015) and registered in ISRCTN number 14115156

Quantitative RT-PCR Total RNA was extracted using PureLink RNA isolation (Life Technologies, 12183025) and treated with DNase (Life Technologies, AM2222)

Composition Of Each Ampoule Of The Dr James Glutathione Injection: Following components are used in the making of Dr James whitening injection: Ascorbic Acid (Vitamin C) 1000mg Alpha Lipoic Acid (Thioctic Acid) 300mg Nano Concentrated Collagen Extract 200mg Epidermal Growth Factor (EGF) 100mg How to Use the Injection