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Cai, Y., Xin, Q., Lu, J., Miao, Y., Lin, Q., Cong, W., & Chen, K

The role of mitochondria in NLRP3 in flammasome activation

After the removal of trace organic solvent, cholesterol esterase was added to detect total cholesterol (free cholesterol and cholesterol esters) following the manufacturers protocol for the Cholesterol/Cholesteryl Ester Assay kit (ab65359, Abcam, Cambridge, MA, USA)

Freezing without a cryoprotectant like glycerol is acceptable for GHK-Cu if single-thaw use is guaranteed, but some researchers add 510% glycerol to the bacteriostatic water during reconstitution specifically to support freeze storage