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In human subjects, several studies suggest that high selenium supplementation increases the risk of type 2 diabetes [103, 104], and, in mouse models, increased expression of selenoproteins as well as selenoprotein deficiency was also correlated with metabolic dysregulation [105]

Quantitative polymerase chain reaction Total RNA was extracted using TRIzol reagent and an Illustra RNAspin Mini RNA Isolation Kit (GE Healthcare, Uppsala, Sweden)

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The above studies also suggest that ROS generation, upregulation of p53, and reduction of mitochondrial membrane potential play important roles in selenium-assisted anti-tumor processes