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Isotopically labeled internal standards included L-carnitine-(trimethyl-d9) (CAS 126827-79-0, Cambridge Isotope Laboratories, Tewksbury, MA, USA), trimethylamine-d9 hydrochloride (CAS 18856-86-5, LGC Standards, Teddington, UK), trimethylamine-d9 N-oxide (CAS 1161070-49-0, LGC Standards), and -butyrobetaine-d9 (CAS 479677-53-7, Santa Cruz Biotechnology, Dallas, TX, USA)

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Example meal: Grilled chicken with quinoa, steamed broccoli, and a drizzle of olive oil

Cellular analysis of the adipose tissue from HFD mice showed that GPNMB high cells, primarily associated with senescent endothelial cells, are significantly reduced due to the effect of the vaccination