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Yan et al., 2020) was used to perform peptide-protein docking to predict the putative binding mode between the designer peptides and loop remodeled GLP-1R

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Western blot analysis and immunoprecipitation Proteins from the cell conditioned media of THP-1 cells were precipitated by the addition of 3 volumes of ice-cold acetone, incubation at 20 C for 18 hours and centrifugation at 13,000 g for 10 min at 4 C

The amount of BAC water varies by vial strength, but 1 to 2 mL is the most common range for peptide reconstitution