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Reduced toxicant yields in mainstream smoke The effect of split-tipping on toxicant yields in mainstream smoke was measured for both volatile and condensed phase toxicants under two machine smoking regimes (ISO, and a higher flow rate 60/2/30 VO smoking regime)

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Cigarette smoke exposure HBE cells were exposed to mainstream smoke from 3R4F University of Kentucky reference cigarettes (Lexington, KY) using a VC-10 Smoking Robot (Vitrocell, Waldkirch, Germany) in accordance with ISO 3308 standards (puff duration 2 s, hold time 0 s, exhaust time 8 s, puff volume 35 mL, frequency 60 s, 6 puffs per cigarette)