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K326, TN 90, and TN 90 SRC were each initially hybridized with an individual plant in which the BBL-a , BBL-b , and BBL-c truncation mutations were initially assembled in triple homozygous conditions ( 1 was then backcrossed to their respective recurrent parents, and selection for all three mutations was carried out using Kompetitive Allele Specific PCR (KASP) markers

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AUD affects both the brain and body, leading to physical dependence, tolerance, and emotional distress when alcohol is unavailable

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