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The incubation system contained 1 mg BSA and 50 l of smoke solution obtained from cigarettes with or without charcoal filter in a final volume of 200 l of 50 mM potassium phosphate buffer, pH 7.4

His own father tried repeatedly to convince him to abandon his political views, but failed, with this only resulting in bitter fights

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The number of years of life lost is calculated for each death which occurs between the ages of 35 and 74 years, and the number is multiplied by the smoking attributable fraction to give an estimate of the number of years of life lost attributable to smoking