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Following centrifugation at 500 g for 20 min at 21 C, the buffy coat was collected, washed twice with PBS, centrifuged again, and prepared for flow cytometry staining

They exert the function of anti-oxidation, anti-lipid production, pro-lipid oxidation, pro-insulin sensitivity, anti-inflammation, anti-apoptosis and antibacterial activities by regulating the main pathways of AMPK/mTOR/NF-B/Stat3, PI3K/Akt/NFB-Nrf2/HO-1, 42, 62, 7 nAChR/AKT/PI3K, 7 nAChR/ERK/NF-B/iB, Akt-p38/Nrf2/HO-1 and target proteins of inflammatory factors IL-6, TNF-, IL-1, IL17, IFN-, IL-8, IL-4, IL-5, IL-1, IL-10, PGE2 (Figure 5)