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That is, dual users reported notably high levels of respiratory symptoms compared to non-users of both cigarettes and e-cigarettes, though an increase in e-cigarette puffs resulted in a marginal decrease in rate of respiratory symptoms

Wang Y, Duan Z, Weaver SR, et al

Afterward, the membranes underwent 3 10 min washes with TBST and were subsequently exposed to secondary antibodies diluted in 0.05% TBST (refer to Supplementary Table S1) for one hour at room temperature

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