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Histone H3 (4499, Cell Signaling, RRID: AB_10544537) was used as a nuclear fraction loading control, and -tubulin (ab7291, Abcam) as the cytosolic fraction loading control

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Cigarette did such a great job on weight savings with the 515 that it added a cabin in front of the helm areasacrilege on most high-performance boats because that extra weight would slow it down