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For the success of an experiment with mainstream tobacco smoke it is important that the distance between the smoke generation (cigarette holders) and the VITROCELL cell exposure system is as short as possible in order to avoid aging and to guarantee a smoke composition matching the real-life situation

THE CHESAPEAKE COLONIES: VIRGINIA AND MARYLAND The Chesapeake colonies of Virginia and Maryland served a vital purpose in the developing seventeenth-century English empire by providing tobacco, a cash crop

doi: 10.1016/j.drugalcdep.2021.109260

Directly after harvest, the leaves were shock-frozen in liquid nitrogen and ground using a mortar and pestle