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PG/VG aerosols increase mucus concentration and MMP-9 activity in sheep in vivo Finally, to determine the effects of PG/VG e-cig aerosols in the airways in vivo, we used our previously established large animal (sheep) model and exposed sheep to 80 puffs per day (40 puffs per session twice daily) of PG/VG e-cig aerosols for five days (Fig
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