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RNA from the barcoded cells was subsequently reverse-transcribed, and sequencing libraries were constructed using a Chromium Single Cell 3 (v3) reagent kit (10x Genomics, PN-1000269) following the manufacturers instructions

These areas include restaurants, schools, day care facilities, hospitals, medical offices and buildings, and places of employment

In particular, benzo[a]pyrene, which is included in the nine WHO TobReg priority smoke toxicants, was reduced by 99.7% in e-cigarette emissions as compared with the smoke from the reference cigarette

Sequence analysis explicates that tospoviruses associated with CMV as Groundnut bud necrosis virus and Capsicum chlorosis virus