Western blotting Cells were subjected to western blot analysis as previously reported [22] using the following antibodies: Nrf2 (PA5-27882, Invitrogen, RRID:AB_2545358), Keap1 (8407, Cell Signaling, RRID:AB_10860776), NF-B P65 (sc-8008, Santa Cruz Biotechnology, RRID:AB_628017), Gpx2 (MA5-36260, Invitrogen, RRID:AB_2890391), Nqo1 (ZRB1707, Millipore Sigma, RRID:AB_3662860), -actin (sc-81178, Santa Cruz Biotechnology, RRID:AB_2223230)
However, there are some more severe side effects that require immediate medical attention
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Dry completely before your child plays with the toy