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The secondary antibody conjugated with the fluorochrome included the donkey anti-goat 488 (1:500 dilution) and donkey anti-rabbit 647 (1:500 dilution) (ThermoFisher Scientific)

In vivo toxicity, biodistribution and clearance of glutathione-coated gold nanoparticles have been extensively investigated 24

This adhesion is mediated through interactions with mucosal lipid moieties and components of the bacterial cell wall, thereby stabilizing the probiotics association with the intestinal epithelium and facilitating sustained hostmicrobe communication (184, 185)

Injection site reactions including minor redness, itching, or irritation occur occasionally