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Cotinine is a specific biomarker of tobacco exposure [1, 2], and the laboratory methods are highly sensitive [17]

The Pennsylvania Department of Health which conducts the Youth Tobacco Survey funded the campaign through a grant, and target areas were identified based on high prevalence of use, heightened exposure to advertising and availability of cessation services

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In a two-way ANOVA model controlling for hemodynamic confounding factors, changes in the overall population remained significant for mitral A a and E a /A a ratio, and for tricuspid A a and E a /A a ratio