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Methods Long-term CSE exposed human bronchial epithelial cell culture BEAS-2B cells were grown in RPMI 1640 (Lonza, Walkersville, MD) supplemented with 15% heat-inactivated Fetal Bovine Serum (FBS), penicillin (100 U/ml) (Lonza) and streptomycin (100 g/ml) (Lonza) on collagen-coated plates

Opioids: Fewer Users, Greater Risk Opioids account for 61 million usersfar fewer than cannabisbut are linked to significantly higher health risks and overdose deaths