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T 1 generation seeds of NtInvVR1 -RNAi plants and their respective controls were surface sterilized with chlorine gas and sown under axenic conditions on Murashige and Skoog (MS) medium including vitamins and MES-buffer (Duchefa Biochemie B.V., Haarlem, The Netherlands), supplemented with 2% Suc (Duchefa Biochemie B.V.), and 0.8% Phyto Agar (Duchefa Biochemie B.V.)

The high nicotine concentration in some brands may cause stomach irritation and nausea