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Discs are hugely important because they act as shock absorbers, cushioning each vertebra from the next, providing spinal stability, proper alignment and enabling movement between vertebral bones

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Thus, to reduce Cd accumulation in the leaves without developmental impairment, it was proposed to combine a nonsense mutation in one of the NtHMA4 homologs, which causes a complete loss of function, with a missense mutation in the other NtHMA4 gene causing functional reduction