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Chromosome Analysis Root tips of viable seedlings were pretreated with distilled water for 24 h at 4C and with 2 mM 8-hydroxyquinoline for 4 h at 4C, then fixed in a 3:1 mixture of ethanol and acetic acid overnight to determine chromosome numbers

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As indicated by consequences of a 23-year long investigation distributed in the Journal Of the Dental Association, stogie smokers experience tooth misfortune and alveolar bone misfortune (bone misfortune inside the jawbone that stays teeth) at rates equal to those of cigarette smokers