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Furthermore, studies focusing on spermatogenesis, a complex biological process regulated by multiple factors 12,13 , have yet to fully elucidate the microenvironmental and transcriptomic variations in the testis resulting from nicotine exposure

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In response to the synergistic stress of heat and drought, tobaccos ecological adaptation mechanisms are primarily reflected in adjusting the plants physiological structure to adapt to adverse environments, such as increasing leaf cuticle thickness and reducing leaf surface area to minimize water loss and altering biochemical pathways to enhance stress tolerance efficiency, such as increasing the activity of antioxidant enzymes, such as CAT and SOD to reduce oxidative stress caused by heat and drought (Carmo-Silva et al., 2012)

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