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In vitro glucose-stimulated insulin secretion (GSIS) Insulin secretion was measured using the Krebs Ringer Bicarbonate (KRB) buffer (119 mM NaCl, 5 mM KCl, 2.5 mM CaCl 2 , 1.2 mM KH2PO 4 , 1.2 mM MgCl 2 , 10 mM HEPES, 25 mM NaHCO 3 , and 0.2% BSA)

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For most people, daily doses of 2,000 mg or less are safe

Mitochondria function as the central effector organelle, translating these regulatory signals into ATP production, reactive oxygen species generation, redox balance, and metabolic flexibility [6]