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Lonza) were seeded onto collagen-coated 12 mm transwell with 0.4 m pore size and differentiated under ALI culture for 28 days in medium with 1:1 mixture of BEBM (Lonza) and DMEM (Gibco) supplemented with 52 g/ml bovine pituitary extract, 5 g/ml insulin, 10 g/ml transferrin, 0.5 g/ml hydrocortisone, 0.5 ng/ml human epidermal growth factor, 0.5 g/ml epinephrine, 1.5 g/ml bovine serum albumin and 15 ng/ml retinoic acid

Seay, the Christian County farmer whos stopped growing the crop, said hes heard companies want to switch to mostly smokeless products, meaning theres likely to be significantly less burley tobacco grown

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