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Reference Fowles44 Several studies have shown that smoking in adolescents and adults with bipolar disorder is associated with earlier age at onset of mood disorder, greater severity of symptoms, Reference Heffner, Anthenelli, Adler, Strakowski, Beavers and DelBello46Reference Medeiros, Lafer, Kapczinski, Miranda-Scippa and Almeida51 poorer functioning, comorbid substance misuse, Reference Wilens, Biederman, Martelon, Zulauf, Anderson and Carrellas52 suicide attempts Reference Ducasse, Jaussent, Guillaume, Azorin, Bellivier and Belzeaux53 and other adverse outcomes, such as readmission to hospital

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The samples were split into three tubes containing 4 mL of lithium heparin, 3 mL of serum, and 2 mL of trisodium citrate, respectively