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Finally, in order to prevent the rebound effect of glutaminase, glutamine is conjugated with phenylacetate to form phenylacetylglutamine, a molecule that cannot be metabolized and is harmlessly excreted in the urine [74]

Patients and caregivers should discuss any neurological symptoms or concerns about vitamin deficiencies with a healthcare provider, as timely diagnosis and treatment can greatly improve outcomes

Various NGS platforms, such as Illumina, Pacific Biosciences, and Oxford Nanopore Technology (ONT), utilize different methodologies, including fluorescently labeled nucleotides and pore-based electrical currents, to improve sequencing accuracy and read length [19]