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The former British Prime Minister created his personal and political image around the cigar and the calm, authoritarian atmosphere it exudes

For a woman once so vilified, it seemed unthinkable that she would ever marry Charles, let alone become Queen

RNA extraction, quantification of miRNA expression and qPCR Total RNA was extracted from the lung tissue samples of the screening cohort and from PBECs derived from non-COPD and COPD patients by TRIzol reagent method (Molecular Research Center, Cincinnati, OH) according to manufacturer's guidelines

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