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Cells were incubated with the diluted DCFH-DA at 37C for 2030 minutes in the dark

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The calcium releaseactivated calcium (CRAC) channels formed by calcium release-activated calcium channel protein (ORAI) 1 and ORAI2 also play essential roles in the non-IgE-dependent activation of MCs within the neuroimmune axis, and they are increasingly being investigated as novel pharmacological targets (170, 171)

The finding of increased nuclear translocation of AIF and EndoG in HBE cells from smokers compared to non-smokers suggests it may play a role in smoke-related lung diseases