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Lung function On the day of the sacrifice, each mouse was anaesthetized intraperitoneally with xylazine hydrochloride (15 mg/kg)/ketamine (100 mg/kg), tracheotomized, and cannulated with an 18 G metal cannula (resistance: 0.360.40 cmH2O.s/mL)
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The analysis consists of a few steps which include sample preparation, microwave digestion, concentration, source preparations, and measurements
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