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Quantitative PCR was performed with SYBR Green PCR Master Mix (Thermo Scientific, USA) according to manufacturers protocol

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Noting that the illicit cigarette trade is substantial here, Singapore Mini Mart Association chair Alan Tay said: These illegal activities have a huge adverse impact on legitimate traders like us, that operate within the parameters of the law. The trade associations are also in the midst of another study on the efficacy and impact on trade of the proposed ban on flavoured tobacco products, and plan to share its results with the Government.