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Interestingly, fat depletion completely reversed the intestinal expression of GST-4::GFP in all sterile worms, including the faint expression in WT worms (Supplementary Figs

Analytical rigor was ensured through LC-MS/MS with isotopically labeled standards, enabling precise quantification of TMA and TMAO across biofluids ( in vitro assays with purified TMA lyase (CutC/D) to clarify inhibition kinetics