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Fold changes in mRNA expression were calculated by normalizing the cycle threshold [C(t)] value of target genes to reference gene S16 using the Ct method

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The use of pharmacotherapeutic smoking cessation aids (NRTs) or prescription medicines for smoking cessation (bupropion and varenicline) was dichotomously categorized into Use and No use. In other words, it was classified as Use when the participant used NRTs or prescription medicines for smoking cessation at least once during the period of the smoking cessation service program, and it was classified as No use when the participant had never used them during the period

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