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To prepare the soft agar colony forming assay, wells of a six-well plate were filled with 2 mL of 0.8% Noble agar (Sigma-Aldrich, USA) in serum-free F-12K medium

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The term is used to describe officers who are overly excitable or energetic, similar to the shows goofy deputy sheriff Barney Fife, played by Don Knotts

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