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(D) Western blots were performed, and (E) the relative protein levels of Sp1, SIRT1, acetyl-HIF-1, HIF-1, p21, and p27 in MLE-12 cells were determined (n = 3)

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[31] Her contemporaries thought she was spoiled by her parents, especially her father, [32] who allowed her liberties not usually permitted, such as staying up to dinner at the age of 13