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The immunoblots were developed with enhanced chemiluminescence (ECL) Plus reagent (Amersham, Arlington Heights, IL) and signals were captured by a Fluor-S MultiImager and quantified using Quantity One software (Bio-Rad, Hercules, CA)

Brand availability may vary slightly depending on: Regional distribution agreements Provincial demand Retail network structure However, federal packaging and labeling requirements apply nationwide

Psychological cravings tied to behavioral habits can persist for several weeks or months beyond that, particularly in response to situational triggers like stress, meals, or social settings