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After incubation for 15 min at 30 C, 1,000 rpm using a 3 mm shaking diameter, cell debris was removed by centrifugation (4,400 g at 4 C for 30 min) and the supernatants were analysed by HPLCMS at two different dilutions (undiluted and four times diluted with 67% methanol, 0.2% hydrochloric acid in H 2 O)

According to the MatTek protocol, on the day of receipt, the EPI-200 skins were conditioned by overnight incubation to release transport-stress related compounds

The tonic NMDA receptor-mediated currents were significantly decreased in morphine-treated mice compared to saline-treated controls (Supplementary Fig

The biosensor surface was blocked with ethanolamine-HCL (1.0 M)