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Each vial requires reconstitution with bacteriostatic water prior to use
Briefly, 400 ]x slices were cultured on (Miiipore, Billerica, MA) for 3 days after which they were biolistically transfected with tomato fluorescent protein (TFP) using a Helios Gen Gun (BioRad, Hercules, CA), according to the manufacturer's protocol, to visualize dendritic arbors
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