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Data analysis Unsupervised principal component analysis (PCA) was first used to observe the overall distribution of samples and the stability of the analysis, followed by supervised partial least-squares discriminant analysis (PLS-DA) and orthogonal partial least-squares discriminant analysis (OPLS-DA) used to distinguish the differences in metabolic profiles

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"This allowed the tobacco industry to make enough profit on premium brands to subsidize its cheap brands, keeping them cheap enough for young people to afford to start smoking and to prevent price conscious smokers from being incentivized to quit." The study analyzed sales data from May 2015 to April 2018