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CAFFEINE Before and during pregnancy High levels of caffeine before and during pregnancy can result in babies having a low birth weight

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distributed by LGC Standards GmbH, Wesel, Germany) were routinely cultivated in Dulbeccos modified Eagles medium (DMEM)/F-12 (11330057, Thermo Fisher Scientific), supplemented with 10 % FBS and penicillinstreptomycin in a humidified incubator at 37 C with 5% CO 2