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To this end, we attempted to form the mixed disulfide bond with purified apo- Pt GSTF8 and its C13S variant using oxidized glutathione (GSSG)

Nonetheless, considering that the half-maximal effective concentration (EC 50 ) for Acetate, Propionate, and Butyrate is around 0.5 millimolar upon both GPR41 and GPR43 (292) and that the SCFA concentration in the human ileum and colon lumen is superior to 100 millimoles per kg (307309), it is likely that both receptors are constitutively active

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