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Briefly, various concentrations (101000 g/mL) of selected compounds were mixed with the mh-Tyr (2000 U/mL) and 5X sample buffer [1.5 M TrisHCl (pH 6.8), 10% glycerol, and 0.01% bromophenol blue] followed by incubation on the ice for 30 min

in this study, measures only the total Trx1 pool that includes Trx1-reduced and Trx1-oxidized, but do not enable identification of Trx1 redox state