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After 1 h, virus was aspirated, and cells were overlayed with carboxy-methyl cellulose (CMC) containing media 0.6% CMC, MEM supplemented with 1 Penicillin/Streptomycin (Gibco), 2% FBS, 1 mM sodium pyruvate (Gibco), 1 NEAA (Gibco), 0.3% sodium bicarbonate (Gibco), and 1 GlutaMAX (Gibco)

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And some infections with EBV are asymptomatic, particularly during childhood. Most Cases of Mono Are Caused by EBV Infection, Which Gets Passed via Saliva If you do come down with mono after an EBV infection, symptoms tend to show up four to six weeks after youre infected with the virus

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