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To further study the dynamics of glucose fluxes in B cell subsets, we measured glucose levels in the culture medium of Gclc fl/fl and Gclc fl/fl Mb1-Cre + FoB after 5 h activation with anti-IgM, CD40 ligand and IL-4

The methods efficacy is highly dependent on the careful optimization of operating parameters, such as ultrasound power, extraction time, solvent type, and temperature, as suboptimal conditions may reduce extraction yields or lead to inconsistent results (41)