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For lipid peroxidation level, Cells were treated with the fluorescent dye BODIPY 581/591 C11 (D3861, Thermofisher Scientific), which inserts into lipid membranes and allows for quantitative assessment of oxidized versus non-oxidized lipids by fluorescing green or red, respectively

Store the vial in its original box if possible, or wrap it in aluminum foil to block light

Of note, the major contributors to MSG neurotoxicity are excitotoxicity, disruption of catecholamine neurotransmission, oxidative stress, activation of inflammatory cascades, and neuronal apoptosis (Seiva et al

ATP release through connexin hemichannels depletes intracellular ATP, leading to necrosis and inflammation (Kalvelyte et al