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Briefly, 100 M assembled double-stranded oligodeoxynucleotide (dsODN) tag was prepared by annealing forward and reverse dsODN oligos in 1x STE buffer (10 mM Tris-HCl, 50 mM NaCl, 1 mM EDTA), heated at 95 C for 5 min and gradually cooled down to 25 C in a thermocycler