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New Engl J Med 382:697705 Krishnasamy VP, Hallowell BD, Ko JY et al (2020) Update: characteristics of a nationwide outbreak of e-cigarette, or vaping, product use-associated lung injury - United States, august 2019-January 2020

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For protein isolation, cells were washed with cold PBS, collected in lysis buffer using a cell scraper, incubated on ice for 20 min, and centrifuged for 5 min at 500 rpm