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Total protein in the homogenates was determined using Micro TP kit (Wako Pure Chemicals, Osaka, Japan)

Tail vein blood (~5-10 l) was collected serially at 0, 30, 60, 90 and 120 min post-challenge through the tail-nick method (19) The blood collection procedure was approved by the Institutional Animal Care and Use Committee of City of Hope in accordance with the NIH Guidelines for Survival Blood Collection in Mice and Rats ( Blood glucose concentration was measured with an Accu-Chek Compact Plus glucometer (Roche Diagnostics Ltd.)

At Cymbiotika, we formulated Liver Health+ to work through multiple pathways