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Other hyphae-inducing media were MM medium supplemented with l-arginine (10 mM), l-glutamate (10 mM), l-glutamine (10 mM), l-proline (10 mM), or N -acetylglucosamine (2.5 mM) at pH 6.5

Proc Natl Acad Sci USA 105:1791317918 Di Giorgio FP, Carrasco MA, Siao MC et al (2007) Non-cell autonomous effect of glia on motor neurons in an embryonic stem cell-based ALS model

Allow each to air dry for 10 seconds before proceeding

While these approaches remain important, a growing body of scientific research suggests that autism may also involve deeper biological mechanisms including mitochondrial dysfunction, chronic neuroinflammation, immune imbalance, and disruption of the gutbrain axis